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Learner Reviews & Feedback for Basics of Extracellular Vesicles by University of California, Irvine

4.7
stars
351 ratings

About the Course

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....

Top reviews

MR

Jan 11, 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

DD

May 17, 2020

I found Ia very professional way to introduce everyone to the amazing world of EV. Professor show elegantly their knowledge. The course fulfils my expectations. Congratulations! Nice works

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51 - 75 of 97 Reviews for Basics of Extracellular Vesicles

By Jess M

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Sep 15, 2016

Aprend铆 mucho. Muy completo, aunque pudieron haber profundizado un poco m谩s.

By Mario N

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Jan 2, 2022

En efecto, bastante b谩sico e introductorio al campo. Muy 煤til para iniciar.

By Alaika D

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Jan 11, 2024

A very good explanation of the topic, describing all the necessary points.

By Rafaela S

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Apr 3, 2024

Excellent course. Short classes, with questions to test your knowledge.

By LIN, T

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Mar 2, 2020

Great course to learn the basic knowledges of extracellular vesicles!

By Petr N

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Oct 10, 2020

Very useful course not only for newbies in the EV field. Thank you

By Daniel A S B

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Apr 4, 2022

It麓s a great course to people that starting to study of vesicles.

By Asmaa A M A

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May 15, 2020

Excellent course, totally enjoyed it and benefited alot from it

By Laura M M E

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May 5, 2020

Excelente curso, expositores con amplio conocimiento del tema

By Stephanie B

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Oct 6, 2019

Good basic overview for someone starting in their studies

By Wenhao L

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Apr 27, 2017

Very wonderful courses! Better than reading 100 papers!

By Mohammad R

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Feb 6, 2017

This course gives an excellent overview of the field!

By Nahla I S H

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Apr 23, 2020

A very good course, really it was very informative.

By pbmansfield

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Mar 7, 2017

Well put together and very informative. Great job!

By Dr.锟� S R S A P -

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Jun 26, 2020

very helpful and nice videos and the contents too

By Julia M

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Jan 13, 2023

Great introduction to EV research and methods.

By Ramiro R d l C

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Nov 5, 2020

Great! Very informative and I learned a lot.

By Emilio E S

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Jul 27, 2021

really good course. I learn a lot about EVs

By Regina C

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Dec 29, 2020

Very interesting and simple to understand

By Olivia C

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Aug 6, 2020

very useful course, I really enjoy it!

By Y. M

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Apr 9, 2020

Very good for beginners.

By PASUT L

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Feb 4, 2023

morally excellent

By David H

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Sep 1, 2020

excellent course

By 脕bner M N

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Nov 22, 2019

Amazing course!

By Julio C T V

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Aug 16, 2022

Excellent!!!!