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Back to Whole genome sequencing of bacterial genomes - tools and applications

Learner Reviews & Feedback for Whole genome sequencing of bacterial genomes - tools and applications by Technical University of Denmark (DTU)

4.6
stars
1,602 ratings

About the Course

This course will cover the topic of Whole genome sequencing (WGS) of bacterial genomes which is becoming more and more relevant for the medical sector. WGS technology and applications are high on international political agenda, as the classical methods are being replaced by WGS technology and therefore bioinformatic tools are extremely important for allowing the people working in this sector to be able to analyze the data and obtain results that can be interpreted and used for different purposes. The course will give the learners a basis to understand and be acquainted with WGS applications in surveillance of bacteria including species identification, typing and characterization of antimicrobial resistance and virulence traits as well as plasmid characterization. It will also give the opportunity to learners to learn about online tools and what they can be used for through demonstrations on how to use some of these tools and exercises to be solved by learners with use of freely available WGS analysis tools . By the end of this course you should be able to: 1. Describe the general Principles in typing of Bacteria 2. Give examples of the applications of Whole Genome Sequencing to Surveillance of bacterial pathogens and antimicrobial resistance 3. Apply genomic tools for sub-typing and surveillance 4. Define the concept of Next-Generation Sequencing and describe the sequencing data from NGS 5. Describe how to do de novo assembly from raw reads to contigs 6. Enumerate the methods behind the tools for species identification, MLST typing and resistance gene detection 7. Apply the tools for species identification, MLST typing and resistance gene detection in real cases of other bacterial and pathogen genomes. 8. Describe the methods behind the tools for Salmonella and E.coli typing, plasmid replicon detection and plasmid typing 9. Utilize the tools for Salmonella and E.coli typing, plasmid replicon detection and plasmid typing in real cases of other bacterial and pathogen genomes. 10. Explain the concept and be able to use the integrated bacterial analysis pipeline for batch analysis and typing of genomic data 11. Demonstrate how to construct phylogenetic tree based on SNPs 12. Apply the phylogenetic tool to construct phylogenetic trees and explain the relatedness of bacterial or pathogen strains 13. Describe how to create your own sequence database 14. Utilize the MyDbFinder tool to detect genetic markers of interest from whole genome sequencing...

Top reviews

MB

Jun 10, 2019

Excellent course with a wealth of information about tools and applications of whole genome sequencing. Presented in a very lucid and interesting manner, very crispy and simplified course.

AP

Jul 20, 2020

Thank you very much for this course through which i learnt basic handling of bioinformatics tools and concepts of WGS. This will help me a lot while pursuing my PhD research work in my field.

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376 - 400 of 419 Reviews for Whole genome sequencing of bacterial genomes - tools and applications

By Babacar N

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May 27, 2022

Super cours avec beaucoup d informations

Merci infiniment

By Anushka G

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Sep 9, 2020

The course was pretty interesting. I enjoyed learning it.

By Ann W

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Sep 27, 2024

A lot of great bioinformatics tools and how to use them

By Carla L I

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May 30, 2020

It has very hard but very interesting for my career

By Dinesh R

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Aug 17, 2020

It was really amazing lecture with detailed demo.

By pacome a

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Nov 30, 2018

perfect course . very instructive and well detail

By ESHA J

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Oct 16, 2020

Thank you so much

This course was really helpful

By Yasser M

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May 5, 2018

It is one of the best MOOC courses I have Taken.

By 502 F

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Feb 20, 2025

Good.. But it could be a credit course

By Rui P d S F M

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Aug 17, 2020

The final task requires too much work.

By Ivonne G

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Nov 20, 2017

I really like it. Easy and practical.

By Suman M

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Nov 1, 2020

good but question are too trickly

By Selcen G Y

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Dec 10, 2024

verilen dosya ekleri a莽谋lm谋yor.

By Lizet L

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Nov 20, 2023

excelente curso, me gusto mucho

By Abarna C

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Sep 9, 2020

The course was interesting!

By Bikash K

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Jan 15, 2019

Very simple and informative

By Romain M

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Jul 23, 2018

Great course, thanks

By Daniel R R

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Sep 19, 2022

exceente curso!!!

By Olga

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Jan 25, 2021

very practical!

By SHUBHANGI K

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May 19, 2025

it is amazing

By Tobi A

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Aug 24, 2025

Very good

By 121922201008 g

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Sep 19, 2020

Great

By Jyotirmayee P

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Nov 21, 2022

good

By MUHAMMAD S A

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Jan 9, 2024

5

By Deleted A

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Jan 18, 2021

I found this to be a very well-structured course which gave me a good overview of a wide variety of tools used in the field. Among other things, I learned how to look for resistance genes and plasmids, and how to determine the species and sequence type of a sample. A sincere thank you goes to all those involved in putting this together.

Each weekly set of lectures is followed by a quiz, but perhaps a few more exercises would have been helpful... something on the lines of the final assignment (an exercise to learn how to interpret phylogenetic trees, for instance). What I think would also have helped is a brief description given by the lecturers of the biological features they were going to talk about - for example, what is the biological meaning of 'sequence type' of a strain? I think this would have made things clearer for beginners.